He received his 2 yellows in the group stage, hence the suspension including getting tossed from the game in which he received the 2nd. The suspension begins immediately. Suspensions don't get wiped out.Actually yellow cards are not cleared for the round of 16, Calafiori got a yellow card today and he'll be missing the game against Switzerland. (round of 16)This is what the Italian sites are saying
EURO 2024 yellow card rules: Which players could be suspended in Germany?
UEFA rules state that players who accumulate a certain amount of yellow cards will miss their country's next game - so who is at risk?
Let's make it simple, if somebody has 1 yellow in the group stage it gets wiped out for the next round. That is the only case where the rule applies.